Where the new SFC Instrumentation adds value.
The recent influx of SFC systems has allowed us to further speed up our already impressive turnaround time for samples.
Two new cutting edge Sepiatec 100s are now our front line preparative systems handling our small to medium scale chiral and achiral SFC separations, leaving the Berger Multigrams free for large scale purifications.
Primary screening is carried out in less than 30 minutes on our Waters UPC2
. Currently over 75% of our SFC separations are found on this system, allowing samples to move to purification within an hour of arriving in the laboratory.
For the other 25% our secondary chiral screen takes place on an Agilent Aurora, consisting of a further nine chiral stationary phases and a range of solvents. Switching valves allow the entire screen to be automated and controlled from the sequence table.
The introduction of two new chiral instruments has allowed the Waters Method Station to become our achiral screen. The column oven is capable of holding ten different columns. We have been carrying out a lot of work regarding achiral SFC recently and we hope to include a roundup of Diki's achiral project over the next few months.