Latest news from Reach Separations - an outsource purification company, that provides chiral and achiral purification from 5mgs to 200 grams. We support all types of companies in discovery, intermediate and the fine chemicals environment.
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Welcome to the moustache-tastic Reach Separations newsletter

This time around we've got some news on our efforts to support Movember by growing our facial hair (except Victoria!)

We've also got an interesting piece on the developments we have made working with achiral SFC - a new orthoganal screening protocol which is producing some good results

As always, if you want more information on anything you've read about feel free to drop us a line or give us a call.

Best regards, Peter Ridgway - Business Development Director (BDD)


This month Reach is yet again raising money for the Movember men's health charity raising awarness (and money!) for testicluar and prostrate cancer. Phil, Duncan, Alex and Pete will all be sporting silly 'tashes throughout november. Victoria will just have to sit this one out as a 'mo-sista'

You can monitor our progress on our photo galleries here where you can also see the results from 2012's attempts.

To sponsor us please visit our official Movember page - thanks.

Achiral SFC

Using SFC for chiral separations is a no brainer, but until recently we hadn't really thought about the practicalities of using SFC for achiral purification.

The benefits of SFC chiral chromatography: fewer salt issues, reduced solvent, faster dry down, higher throughput, faster column equilibration, can all be applied to achiral samples too. The only negative when compared to reverse phase is the lack of a C18 equivalent 'one column certainly doesn't fit all'.

The bench mark in achiral reverse phase is C18. We turn to this phase immediately when starting method development work. Unfortunately there is not an equivalent when it comes to SFC and after extensive reading and searches, looking at 2-EPs, Silicas, Hilics and Nitros we found ourselves back at the beginning when trying to choose a column. So we chose them all!

With a newly stocked suite of 10 Achiral SFC columns and a few standards under our belt, we were up and running, waiting for our first 'simple' real sample to purify by SFC as an alternative to reverse phase. Then it arrived...

Real? Yes! Simple? No!

Our first achiral sample for purification by SFC . . a multi component mixture! And this separated on bare silica...


The chromatogram above shows the multicomponent separation run on a 5-50% gradient over 16 minutes. With most SFC's designed for chiral separations, an open bed fraction collector was not available to us. We had 4 collection vessels. Therefore splitting the initial mixture into 4 and isolating the main peak and latest eluting peak gave us two clean peaks and two further mixtures which we were then able to method develop further.

4 Days later and...

Our first real go at achiral SFC was tricky but successful. We were certainly more than grateful of the lack of water to evaporate considering this sample required several passes. Since then we have processed a range of samples from Ultrapure to impurity isolations with the same level of success.

So this is the headline from our initial entry to achiral SFC; the drawback of the extended screening and method development time are more than balanced by the reduced post processing time and faster methods. SFC seems to be a winner for achiral samples too.

Movember logo

UKASF - 2013

Reach Separations are attending the UKASF meeting hosted at Biofocus on 12th November - if you are attending have a wander around the exhibition and say hello. We'll also be presenting a short talk on our updated capabilities.

Ich Bin Ein Berliner

Our Business Development Director Peter Ridgway will be presenting at the users group meeting in Berlin on 13th November - surely the highlight of the meeting and one not to be missed!
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